Western blot. All lanes: GABPB1antibody at 1.11ug/ml. Lane 1: Rat gonadal tissue. Lane 2: Hela whole cell lysate. Lane 3: HT29 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 42, 39, 37 kDa. Observed band size: 43 kDa.