Western blot. All lanes: ESR1 antibody at 7µg/ml. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: Rat brain tissue. Lane 4: Colo320 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 67, 54, 48, 36 kDa. Observed band size: 67 kDa