Western blot. All lanes: 14-3-3 protein theta antibody at 4ug/ml. Lane 1: A549 whole cell lysate. Lane 2: Mouse lung tissue. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa.