Western blot. All lanes: XIAP antibody at 3µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: PC-3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa