Western blot. All lanes: TMEM19 antibody at 1µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: Mouse stomach tissue. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 33 kDa. Observed band size: 37 kDa