Western Blot. Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, Rat brain tissue, Rat liver tissue, Mouse liver tissue, Mouse brain tissue. All lanes: TH antibody at 4ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 56, 57, 46, 45 kDa. Observed band size: 59 kDa.