Western blot. All lanes: Tyrosyl-DNA phosphodiesterase 1 antibody at 8ug/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Mouse lung tissue. Lane 3: Rat brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 69, 42 kDa. Observed band size: 69 kDa.