Western blot. All lanes: SIGLEC6 antibody at 6µg/ml. Lane 1: Human high value serum. Lane 2: A549 whole cell lysate. Lane 3: Caco-2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50, 39, 49, 38, 43, 45 kDa. Observed band size: 50 kDa