Western BlotPositive WB detected in: U251 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293T whole cell lysate(20µg), A549 whole cell lysate(20µg),Mouse Brain tissue lysate(20µg)All lanes: OVOL1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 31 kDaObserved band size: 31 kDaExposure time: 30s