Western blot. All lanes: NFKBIE antibody at 4.17ug/ml. Lane 1: Mouse liver tissue. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: A375 whole cell lysate. Lane 5: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 53 kDa. Observed band size: 53 kDa.