Western blot. All lanes: NAA20 antibody at 10µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Rat brain tissue. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 21, 13 kDa. Observed band size: 21 kDa