Western blot. All lanes: MED7 antibody at 6µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse thymus tissue. Lane 4: NIH/3T3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa