Western blot. All lanes: MAPK15 antibody at 6ug/ml. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60, 29, 32 kDa. Observed band size: 60 kDa.