Western blot. All lanes: KLC1 antibody at 1.34µg/ml. Lane 1: Colo320 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 66, 64, 65, 72, 70, 67, 63, 73 kDa. Observed band size: 72 kDa