Western blot. All lanes: HCCS antibody at 3.54µg/ml. Lane 1: Mouse small intestine tissue. Lane 2: Mouse heart tissue. Lane 3: Mouse skeletal muscle tissue. Lane 4: Hela whole cell lysate. Lane 5: RAW264.7 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lane 7: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 31 kDa. Observed band size: 31 kDa