Western blot. All lanes: Transcriptional repressor CTCF antibody at 6ug/ml. Lane 1: PC-3 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 83, 46 kDa. Observed band size: 83 kDa.