Western blot. All lanes: COIL antibody at 3ug/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa.