Western blot. All lanes: Circadian locomoter output cycles protein kaput antibody at 6ug/ml. Lane 1: Hela whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 95 kDa. Observed band size: 95 kDa.