Western blot. All lanes: ATP6AP2 antibody at 10µg/ml. Lane 1: Mouse eye tissue. Lane 2: COLO205 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 40, 36 kDa. Observed band size: 40 kDa