Western blot. All lanes: Afp antibody at 10µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Rat liver tissue. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 68 kDa. Observed band size: 68 kDa