Western blot. All lanes: ACTL6A antibody at 4 ug/ml. Lane 1: Mouse thymus tissue. Lane 2: Mouse spleen tissue. Lane 3: Mouse heart tissue. Lane 4: Jurkats whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: Hela whole cell lysate. Lane 7: A549 whole cell lysate. Lane 8: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48, 44 kDa. Observed band size: 48 kDa.