All lanes:Mouse anti-Human Cyfra21-1(KS19.1) monoclonal antibody at 4ug/ml. Lane 1: MCF7 cell lysate. Lane2: MDA-MB-231 cell lysate. Lane3: HepG-2 cell lysate. Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution. Predicted band size : 30 kDa. Observed band size : 32 kDa. Additional bands at: 50kd.