Western Blot. Positive WB detected in: 10ng Flag Tag fusion proteinFlag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa.