Calbindin D-28K Antibody (4H7)
Product Details
- Cat. No.
- NBP2-50048
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/11d8af622abc4b188f6b4ddb7af681d18d8ab03d-400x204.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/11d8af622abc4b188f6b4ddb7af681d18d8ab03d-400x204.jpg?w=256&q=75&fit=clip&auto=format)
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Description
Calbindin D-28K Antibody (4H7)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Bovine, Human, Mouse, Pig (Swine)
- Antigen: Calbindin D-28K
- Isotype: IgG1
- Source: Affinity purified
Handling
- Quantity: 0.025ml
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS with 50% glycerol
Applications
- Immunocytochemistry (ICC)
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/11d8af622abc4b188f6b4ddb7af681d18d8ab03d-400x204.jpg?w=3840&q=75&fit=clip&auto=format)