ATG13 [p Ser318] Antibody
Product Details
- Cat. No.
- NBP2-19127
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f0955b8cfb3ee1267fd79c9b6e5cb28ba0a69975-400x300.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.
![Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f0955b8cfb3ee1267fd79c9b6e5cb28ba0a69975-400x300.jpg?w=256&q=75&fit=clip&auto=format)
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Description
ATG13 [p Ser318] Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human
- Antigen: ATG13
- Modifications: p Ser318
- Source: Immunogen affinity purified
Handling
- Quantity: 0.1 mg
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Applications
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Western Blotting (WB)
![Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f0955b8cfb3ee1267fd79c9b6e5cb28ba0a69975-400x300.jpg?w=3840&q=75&fit=clip&auto=format)