The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992)
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Amphibia, Bovine, Chicken, Human, Monkey
- Antigen: Vimentin purified from bovine lens
- Clone: VIM 3B4
- Conjugate: unconjugated
- Isotype: IgG2a kappa
- Quantity: 50 µg
- Storage: short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)
