The antibody stains specifically the nucleolus and Cajal bodies in interphase cells. The antigen is a constitutively expressed nucleolar component and a specific marker for the dense fibrillar component of the interphase nucleolus. The protein recognized is a 180 kD polypeptide in Xenopus (xNopp180), homologue to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Xenopus
- Antigen: nucleolar fraction prepared from Xenopus laevis oocytes
- Clone: No-114
- Conjugate: unconjugated
- Isotype: IgG1
- Quantity: 5 mL
- Storage: short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blotting (WB)
