Anti-Human IgE polyclonal antibody
Product Details
- Cat. No.
- DPAB1249SH
- Clonality
- Polyclonal
- Host
- Sheep
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Description
Sheep anti-Human IgE polyclonal antibody for RID, DID, IEP, IHC, ELISA.. Sheep Antibody to Human Immunoglobulin E (IgE), Epsilon Chain Applications：Suitable for use in a variety of gel techniques including RID, double diffusion, IEP, and rocket IEP. The useof 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in immunohistochemical procedures, enzyme-linked immunoasorbent assays and Western blot has not been assessed but use in such assays should not necessarily be excluded. RID: 5ul antiserum/cm2 in gel vs 5ul IgE myeloma (1mg/ml) Neat - 1:100. Double Diffusion: 10ul antiserum vs 1ul myeloma sera (1mg/ml). IEP: 100ul antiserum vs 5ul IgE myeloma sera (1mg/ml)
Biological Information
- Clonality: Polyclonal
- Host: Sheep
- Reactivity: Human
- Conjugate: unconjugated
- Gene: 3497
Handling
- Storage: Upon receipt, store at 2-8°C. Slight precipitation can °Ccur on storage which may be removed by centrifugation, and should not affect performance characteristics
- Buffer: Glycine buffered saline, pH 7.4
Applications
- ELISA (ELISA)
- Western Blotting (WB)