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Detection of Breast Cancer-Related Germline Mutations with Advanced NGS Technology

13 Aug 2019
Detection of Breast Cancer-Related Germline Mutations with Advanced NGS Technology

As breast cancer remains the most common tumor in women worldwide, identifying relevant germline predisposing mutations is important in determining a patient’s lifetime risk of developing breast cancer and allows early detection, clinical management and treatment. Through advances in next-generation sequencing technology, Dr. Valeria D’Argenio, Federico II University and CEINGE Biotecnologie Avanzate, Italy, discusses data from six years of experience in hereditary breast cancer diagnosis using BRCA amplicon sequencing and custom panel analysis.

Agilent Solutions for Automated NGS Sample Prep

Agilent Technologies

Agilent Solutions for Automated NGS Sample Prep provide: Flexibility to automate virtually any NGS reagent Performance to dramatically increase throughput and walk-away time Optimized SureSelectXT protocols to rapidly automate your NGS Sample Prep Agilent NGS Option A and Option B Both Deliver Higher Throughput with Less Hands-On Time Automating your NGS sample preparation with either Agilent NGS Option A or Option B increases laboratory throughput up five-fold compared to manual sample preparation. For whole genome sequencing applications that require only library preparation, 96 samples can be prepared in less than 4 hours to increase laboratory output to 960 sequencer ready libraries in a single week. For more complex protocols, such as targeted resequencing using Agilent SureSelectXT, both systems can complete library preparation and target enrichment for 192 samples in a 5-day work week. Agilent NGS Option A: High throughput and improved reproducibility with a small footprint Agilent Bravo Automated Liquid Handling Platform Agilent PlateLoc Thermal Microplate Sealer Agilent Bravo accessories for heating, cooling, shaking, and magnetic separation Agilent NGS Workstation Option B: High throughput and improved reproducibility with more walkaway time Agilent NGS Option A Agilent BenchCel 4R Microplate Handler Agilent Labware MiniHub

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Detection of Breast Cancer-Related Germline Mutations with Advanced NGS Technology