Scaling the potential of patient-derived organoids through automation and label-free image analysis of individual organoids

3D patient-derived tumor organoids hold great potential for many basic and translational applications. However, traditional approaches to organoid image analysis and downstream data processing are often challenging and labor-intensive.

In this presentation, Dr. Christophe Deben, Group Leader of the University of Antwerp’s Tumoroid Screening Lab, demonstrates a semi-automated workflow for cell seeding drug treatment using the Tecan D300e digital drug dispenser and live-cell imaging using the Tecan Spark^® Cyto* multimode plate reader. In addition, Deben illustrates the development and integration of label-free brightfield image analysis software (OrBITS) to monitor organoid growth rates and track movements.

This talk was presented, in partnership with Tecan, at SLAS2023.

*Spark^® Cyto is for research use only. Not for use in diagnostic procedures.