28 June 2017
Real-time monitoring of phagocytosis using the IncuCyte® live-cell imaging & analysis system. Time-lapse phase contrast and fluorescence images, acquired using IncuCyte instrument, enable the real-time visualisation of IncuCyte™ pHrodo® Green E. coli Bioparticles® engulfment by the mouse macrophage cell type J774A.1. The Bioparticles are phagocytosed and enter the acidic environment of the phagosome resulting in increased fluorescence of the pHrodo® label. IncuCyte integrated image analysis tools enable detection and measurement of the green fluorescent signal over the entire assay time-course and eliminate the impact of background fluorescence.