LGC Genomics Webinar: oKtopure & Genespin - DNA Extractions Made Easy

  The Genespin™ platform completes the nucleic acid extraction range has been engineered to fulfill the growing demands for the highest throughputs.   Speed is of the essence when it comes to DNA analysis of plant breeding populations; whole generations must be analysed for the presence or absence of desired biomarkers in time to enable the correct plants to be used in the next generation of breeding. This creates a demand for high-throughput DNA extraction from plant tissues, with resultant purified DNA of a suitable quality for PCR-based applications. The Genespin™ enables semi-automated but high throughput DNA extractions from plant tissues, and utilises the proprietary Kleargene™ extraction chemistry.   The Kleargene spin plates utilise a glass fibre solid support inserted into a microtitre plate, in 96- and 384-well formats. To guarantee a throughput of more than 5000 extractions per day, the Genespin has been optimised to load the buffers into the spin columns without any cross-contamination. Kleargene kits are available in both 96-well and 384-well formats and are suitable for extraction of between 100ng and 5 µg DNA from plant material. In conjunction with our SNPline™, the Genespin platform provides DNA of a quality that is sufficient for downstream applications including PCR SNP genotyping including KASP, andarray technologies.   Features: Enables high throughput DNA extraction: 8 x 96 plates/ hour. - 96 format: up to 5,500 samples per day. - 384 format: up to 20,000 samples per day Minimise consumable costs: in-built tip wash station ensures that only one set of tips is required per run Replicator function: the Genespin instrument can be used to prepare DNA dilution plates (96- and 384-well) and to stamp DNA into PCR plates Efficient use of laboratory space. - Small format: 170 cm x 68.5 cm x 65 cm Compatible with existing SNPline installations  

The oKtopure™ is a fully automated nucleic acid extraction instrument that enables a new level of high-throughput processing and significant cost savings. This platform combines standardised high-throughput DNA or RNA extraction from a variety of sample types with the proprietary sbeadex™ magentic bead based extraction chemistry. The oKtopure is part of our on-going programme to provide customers with high productivity solutions, first developed and optimised for use within our service labs, where every day we deliver Extraction, Sequencing and Genotyping services. Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads, and hence the DNA in place whilst transferring the buffers required for washing, binding and elution steps. The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping, NGS and Sanger sequencing. Features: High throughput - capable of processing eight 96-well plates (768 samples) in approximately one-and-a-half hours Robust tip washing system reduces consumable costs by 50% or more Preloaded methods for different sample types – fully customizable Extraction expertise and support from scientists who use the instrument daily and have been carrying out high throughput extractions since 1996!