Energy Validation - ECAR/Lactic Acid Correlation

1 March 2016

 Generation of an A549 knockdown for fumerate hydratase, creating a Krebs cycle deficient line, and an A549 knockdown for both fumerate hydratase and lactate dehydrogenese A, creating a glycolysis deficient line. Lactate accumulation and ECAR were then measured ina colormetric assay and using the XF analyzer respectively.