Cloning with Restriction Enzymes
19 May 2014
Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.
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NEB RE-Mix® Restriction Enzyme Master Mixes
New England Biolabs Inc.Restriction enzyme digests are now even easier!The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add your DNA and digest for 15 minutes.Available Mixes: AgeI-HF® RE-Mix® OrderAscI RE-Mix® OrderBstEII-HF® RE-Mix® OrderEcoRI-HF® RE-Mix® OrderEcoRV-HF® RE-Mix® OrderKpnI-HF® RE-Mix® OrderMfeI-HF® RE-Mix® OrderNcoI-HF® RE-Mix® OrderNheI-HF® RE-Mix® OrderNotI-HF® RE-Mix® OrderPacI RE-Mix® OrderSalI-HF® RE-Mix® OrderScaI-HF® RE-Mix® OrderSpeI RE-Mix® OrderXbaI RE-Mix® OrderXhoI RE-Mix®
Restriction Enzymes
New England Biolabs Inc.Presently, NEB supplies more than 200 restriction enzymes, over 120 of which are available in recombinant form.
