29 June 2023
In this video, CURIOSIS demonstrates the monitoring of concentration-dependent spheroid formation in two cell lines, MDA-MB-231 and Hela cells, using the Celloger® Mini Plus. With a 4x objective and green fluorescence, the system captured the differences in spheroid size between the two cell lines, despite having the same number of cells forming the spheroids. Hela cells occupied a smaller area and showed more effective spheroid formation, suggesting stronger cell aggregation compared to MDA-MB-231. The cells were seeded at different densities (10,000, 5,000, and 1,000 cells per well) in a round-bottom 96-well plate and cultured for 26 hours.