Assay for Transposase Accessible Sequencing (ATAC-seq) at Emory University
23 Dec 2015
Watch this video Dr Chris Scharer of Emory University to hear how the recent description of the Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) has transformed the analysis of gene regulation by allowing highly efficient generation of sequencing libraries that map regions of DNA accessible to proteins, thus identifying active promoters, enhancers, and other cis-regulatory elements. The accessibility of DNA to proteins such as transcription factors and polymerases has long been a surrogate for regulatory activity.
Related Products
Request Quote for All Products
KAPA Library Quantification Kits
Kapa Biosystems, Inc.KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries. The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency. Features: Accurately quantify PCR-competent sequencing templates qPCR only “counts” those library molecules that can be sequenced More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources Improve pooling for multiplexed sequencing Library concentrations determined by qPCR are more reliable compared to other methods The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts Eliminate drift with carefully quality-controlled DNA Standards Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time Improve throughput with automation Library quantification assay is compatible with 96- and 384-well format Library dilution, reaction setup and data analysis can be automated for HTP pipelines Applications: Quantification of libraries prior to pooling for multiplexed sequencing Quantification of individual libraries or library pools prior to cluster amplification or emPCR Quantification of libraries prior to and after hybridization capture Quality control, optimization and troubleshooting of library construction processes or workflows
