SelectScience InterviewsLife Sciences
A step-by-step workflow for a knock-out experiment in iPSCs
19 Dec 2023![A step-by-step workflow for a knock-out experiment in iPSCs A step-by-step workflow for a knock-out experiment in iPSCs](https://cdn.sanity.io/images/f5b6mtfn/selectscience-prod/4cd978a92d531ca3dac8f27354ecba6ddd4926b8-805x450.jpg?w=3840&q=75&fit=clip&auto=format)
This step-by-step tutorial video demonstrates the process of conducting a gene knock-out experiment in induced pluripotent stem cells (iPSCs) using the Neon™ NxT Electroporation System. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; isolate and clonally expand the culture; and validate the resultant stem cells. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series: