The use of high-throughput flow cytometry and bio-layer interferometry in virus research
21 Jul 2022
This webinar will consist of two presentations from Public Health England (PHE) and The Pirbright Institute with regards to the role of high-throughput flow cytometry and bio-layer interferometry in virus research, respectively.
Measuring antibody-dependent complement deposition to assess immunity to SARS-CoV-2
As the world reeled from the impact of the COVID-19 pandemic, it was critical that laboratory assays were developed to aid understanding of natural immunity and to assist in vaccine design and testing. While the presence of neutralization antibodies has been the presumed correlate of protection from infection, other functions of antibodies have been linked to protection from severe disease. In this webinar, we will discuss the challenges of developing and running a magnetic bead-based antibody-dependent complement deposition assay on the iQue® platform, and show its utility in measuring functional immunity in convalescent and vaccine samples.
Investigating the avidity of antibodies against the capsid of foot-and-mouth disease virus using bio-layer interferometry
Foot-and-mouth disease (FMD) is a high consequence viral disease of clove-hooved livestock caused by the FMD virus (FMDV), with vaccine afforded protection largely correlating with antibody titer. However, this relationship is not absolute and there is an increasing appreciation of the role played by antibody avidity. Thus, characterizing antibody avidity has important consequences with regards to vaccine development. In this webinar, discover how bio-layer interferometry was used to identify the avidity of monoclonal and polyclonal antibodies to the surface of FMDV particle. Importantly, as well as a peptide mimicking a receptor-binding loop, recombinantly expressed virus-like particles (VLPs) were used to display the entire viral epitope landscape and reflect the complete repertoire of antibody virus binding.
Who should attend?
COVID-19 and infectious disease researchers, vaccine developers in biopharma/pharma and biotech, and academic institutions, government and environmental agencies, and labs primarily working on testing, R&D, vaccine research and virology
Job titles: R&D employees, Research scientist, Research director, VP research, Staff scientist, Senior scientist, Technician, Research assistant, Facility director, Laboratory director/head, MD, Chief Scientific Officer, Post-doctorate, PhD, Professor
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.