Optimizing cryopreserved tissue samples using cell sorting to study cellular heterogeneity
08 Dec 2022
The availability of fresh human tissue remains an impediment to the study of solid tumor indications. The generation of a highly-annotated™ cryopreserved dissociated tissue biobank provides the opportunity to develop highly focused study cohorts to understand the complex cellular heterogeneity of human tissues. Performing “-omics” analyses on heterogeneous biological tissue samples can, however, lead to biased results, particularly when the target cells are present in relatively low numbers.
Join this webinar to understand how Discovery Life Science’s cell services laboratory processes primary human tissue for downstream analytical evaluation. Sony’s MA900 cell sorter is an integral part of multiple workflows that support both the analyses of cell populations and preparation of specimens for multiple downstream applications. Experimental data showing single cell resolution of protein expression and results using 10x Genomics single cell RNA-seq (scRNA-seq) technology will be discussed.
- Learn how the dissociation and cryopreservation of primary solid tissues can accelerate preclinical pipelines
- Review the workflow used by Discovery that combines cell sorting, cellular debris clean up, and immunophenotyping of primary human tissue into one step to generate single cell data
- Discuss the latest approaches such as 10x Genomics and custom cell culture studies used in downstream single cell applications
Who Should Attend:
This webinar will provide insights to researchers who want to learn how to utilize cryopreserved primary solid tissues to accelerate preclinical pipelines. Attendees will learn how to optimize the samples from biobanked tissue using cell sorting to perform single cell transcriptomics studies.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.