NMR for understanding functional cellular pathways: Metal transport and homeostasis
09 Dec 2020
NMR spectroscopy can provide unique contributions for the description of cellular processes, as it is indeed suitable not only for characterizing the structural and dynamical properties of biomolecules but also for describing transient interactions and functional events with atomic resolution possibly in a cellular context. This requires the development of suitable methodologies capable of addressing multiple, specific, and sometimes non-conventional aspects for describing functional processes in cells.
In this webinar, Prof. Lucia Banci, University of Florence, describes how the power of NMR can be harnessed for describing cellular pathways at atomic resolution in a cellular environment. Prof. Banci will present examples for pathways responsible for cellular copper trafficking and for the biogenesis of iron-sulfur proteins.
Through an integrated approach, by increasing the complexity from single protein structures to protein complexes to the functional reaction steps, these processes will be described in their cellular context within a molecular perspective. Furthermore the exploitation of UHF NMR spectrometers, such as that operating at 1.2GHz for in cell NMR applications will be discussed.
The webinar will be introduced by Dr. Rainer Kuemmerle, Vice-President at Bruker Biospin and Head of NMR Applications, who will briefly talk about the development of the world-first NMR system operating at 1.2GHz magnetic field installed at University of Florence and answer questions related to this technology.
Key learning objectives:
- How NMR spectroscopy can provide unique contributions for the description of cellular processes
- How NMR can be used to describe transient interactions and functional events with atomic resolution
- How NMR is a unique tool for characterizing metal binding protein structure and dynamics
Who should attend
Anyone interested in learning how in-cell NMR at ultra-high fields can be used to monitor protein function