NGS sample quality control: Comparison of different methods to isolate high-molecular weight DNA from bacteria for Nanopore sequencing
28 May 2021
Nanopore long-read sequencing has overcome many of the restrictions of short-read sequencing, enabling the resolution of repetitive regions and the assembly of closed bacterial genomes.
This has allowed more researchers to make use of long-read technologies for a variety of applications. Besides the careful handling of samples in library preparation, the most important prerequisite to achieve optimal sequencing results is the quality and size of the input DNA. Size distribution has a major impact on the read length and therefore the quality of the assembly and success of resolving large repetitive regions. The proper isolation of high-quality DNA of optimal size is therefore crucial for successful Nanopore sequencing.
The German NGS Competence Center in Tübingen tested different DNA isolation kits from different manufacturers mostly dedicated for high-molecular weight DNA isolation and performed quality control analysis using the Agilent Femto Pulse system to determine the size and distribution of the isolated genomic DNA.
Key learning objectives:
- How to achieve optimal sequencing results by determining quality and size of input DNA
- Why proper isolation of high-quality DNA is crucial for successful Nanopore sequencing
- How factors such as yield and purity influence sequencing data results
Who should attend?
- NGS and genomics researchers
- Core Lab / Shared Services laboratory managers / technicians
- Sequencing lab managers/technicians
- Molecular biologists
- Cancer researchers