Multiplexing in real-time PCR - benefits, challenges, and opportunities

With real-time PCR, the amplification of PCR products can be monitored in real-time using fluorescent dyes. This method, often referred to as quantitative PCR (qPCR), is utilized for precise quantitative analysis. To maximize information obtained from a single sample, multiplex PCR is a widely employed molecular technique capable of amplifying multiple targets in a single PCR experiment. It has proven to be invaluable for identifying biological parameters and monitoring gene expression in diverse organisms, combining multiple PCR assays into one, allowing for efficient use of sample material and reducing well-to-well variation. While the targets are amplified simultaneously, they are independently detected using reporters with distinct spectra.

In this webinar, Dr. Ines Leonhardt and Melanie Jahn from Analytik Jena will demonstrate the advantages of multiplexing in real-time PCR and discuss various challenges and opportunities associated with this multiplex qPCR technique.

Key learning objectives

• Learn more about the advantages and challenges in using multiplex qPCR
• Explore general tips to start multiplexing in qPCR
• Discover the role of color compensation in qPCR
• Explore examples for multiplex qPCR applications

Who should attend?

• General users of qPCR
• Researchers interested in learning about multiplex qPCR
• Scientists already working with multiplexing and who want to expand their understanding

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.