Leveraging the power of core-shell LC particles to improve micro- and nanoflow separations
25 May 2021
The use of micro- or nanoflow separation is a powerful tool to increase sensitivity in LC-MS workflows. The increase in sensitivity is of great importance in application areas where one has a limited amount of sample, such as bottom-up proteomics. However, it can also be leveraged to allow for the use of smaller sample sizes when sample collection is intrusive, such as biological samples, and needing to harvest less sample is a major benefit.
The sensitivity improvements seen in micro- and nanoscale separations are afforded by the increased ionization efficiency when less mobile phase is present in the analyte band. This sensitivity increase, however, can be further improved by having higher efficiency columns that generate narrower peaks. As the chromatographic peak width reduces, the height increases, leading to sensitivity improvements. Better analyte resolution also reduces the duty cycle of the MS, providing better spectral information.
In this webinar, Jason Anspach, from Phenomenex, will demonstrate how the use of core-shell-based HPLC materials, long known to significantly improve efficiency in analytical HPLC, can be used in micro- and nanoscale LC-MS analysis to improve separation quality and provide better sensitivities.
Key learning objectives:
- An introduction to using micro- and nanoflow to increase sensitivity
- How to implement core-shell LC particles for additional performance, identification, and speed benefits
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.