How to streamline the assay development of a cytotoxicity assay for high-content compound screening
23 Feb 2021

High-content screening and morphological profiling is becoming increasingly popular in the characterization of new compounds and their mode of action in pharmaceutical and academic drug development. Using highly sensitive technologies, morphological changes can already be detected in sub-cytotoxic concentrations. For the generation of morphological profiles, a precise investigation of cytotoxicity with respect to concentration and time is therefore indispensable.

In this webinar, Dr. Christopher Wolff, FMP Berlin, presents a fast and reliable method to develop a cytotoxicity assay for high-content screening using Tecan’s Spark® Cyto multimode reader with imaging capabilities. The imaging-based apoptosis assay enables a precise determination of the apoptotic state in treated cells. The Spark® Cyto provides highly sensitive and precise characterization of compound-induced cytotoxicity, while multicolor image analysis allows for rapid and reliable detection of early and late apoptotic cells. The assay performance was found to be comparable to dedicated high-content screening imagers, enabling a rapid integration of this assay into large-scale high-content screens.

Key learning objectives:

  • Learn how to streamline assay development for HCS applications by applying a multimode reader with imaging capabilities
  • Learn about the multiplexing capabilities of Tecan’s Spark Cyto plate reader
  • Discover how parallel image acquisition and analysis can speed up your assay development process

Who should attend?

  • Professional groups: Ph.D. students, postdocs, general investigators, senior scientists, lab-heads
  • Research areas: Cell biology, oncology, drug development, high content/throughput screening, cytotoxicity, apoptosis
  • Technologies of interest: Multicolor fluorescence imaging, live-cell imaging, image analysis

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary for continuing education purposes.