A multi-platform metabolomics approach for exposure science using mass spectrometry
16 Dec 2021

New technologies and methods are continually pushing the field of metabolomics by expanding the metabolite landscape interrogated within a single sample. One exciting approach is to exploit and integrate the orthogonality of both liquid chromatography (LC) and gas chromatography (GC), coupled to high-resolution mass spectrometers, into a metabolomics workflow. In this presentation, Dr. John A. Bowden, Assistant Professor in the Department of Physiological Sciences in the College of Veterinary Medicine at the University of Florida, will examine a diverse suite of reference materials using a multi-platform metabolomics approach. The utility of the multi-platform metabolomics approach will be explored for the analysis of a novel in vitro gastrointestinal model to assess the effects of ubiquitous chemicals on the microbiome. Measurement of emerging volatile per- and polyfluoroalkyl substances (PFAS) will also be discussed.

Key learning objectives:

  • Learn how to implement metabolomics experiments for both gas chromatography and liquid chromatography mass spectrometry using the Thermo Scientific Orbitrap Exploris GC 240 mass spectrometer and Thermo Scientific Q-Exactive Orbitrap mass spectrometer
  • Learn how high-resolution accurate-mass data can provide broader coverage and a deeper understanding of samples, including detection of merging compounds
  • Learn how in vitro techniques can be used to assess the effects of ubiquitous chemicals on the microbiome

Who should attend?

  • Research scientists with a focus on metabolomics, exposomics, and emerging contaminants
  • Analytical chemists
  • Laboratory managers
  • Gas and liquid chromatography-mass spectrometry users

Thermo Fisher Scientific