A deep dive into OriginPro for the analysis of high-resolution DGE-AUC data
04 Apr 2023

Analytical ultracentrifugation (AUC) has been a benefit to the field of biophysical characterization for nearly a century. Sedimentation velocity (SV) AUC has emerged as a gold standard for quantifying the loading fraction of gene therapy delivery particles such as adeno-associated viruses (AAV) and liposomes. However, boundary analysis of SV-AUC data requires specialized software such as Sedfit or UltraScan.

In this joint webinar with OriginLab corporation, density gradient equilibrium (DGE) AUC will be introduced – an orthogonal technique that can be conducted in the same Optima AUC instrument as SV-AUC. DGE-AUC offers improved sensitivity and sample consumption, along with dramatically simplified analysis. The webinar will also demonstrate a simple, expert analysis of DGE-AUC data in OriginPro* software.

*Third-party analysis software, including UltraScan, Sedfit, and OriginPro has not been validated by Beckman Coulter Life Sciences for use with the Analytical Ultracentrifuge. Beckman does not endorse any third-party analyses software. Beckman Coulter Life Sciences warranty and/or performance guarantee that may be applicable or are provided by Beckman for Analytical Ultracentrifuge do not apply to any third-party software.

Key learning objectives

  • Learn about the advantages of DGE-AUC for your characterization suite
  • Understand how to start running DGE-AUC experiments on viral vectors like AAV and adenovirus (AdV)
  • Discover rapid and optimal workflows for analyzing DGE-AUC data in OriginPro* including importing, peak fitting, calculations, and more

Who should attend?

Anyone characterizing viral vectors

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Beckman Coulter Life Sciences