Real-time, at-line cell culture media analysis of complex samples

Friday, September 12, at 15:00 BST | 16:00 CEST | 11:00 EDT | 08:00 PDT

Modern cell culture media and feeds often contain over 50 to 100 components, including amino acids, sugars, vitamins, trace metals, and buffers, making it difficult to accurately measure amino acid concentrations. In fed-batch and perfusion processes, where nutrient levels fluctuate dynamically, real-time insights are critical for optimizing feed strategies and accelerating upstream development. Traditional methods like outsourced capillary electrophoresis-mass spectrometry (CE-MS) often result in data delays of several days.

Yet, accurate amino acid profiling is essential for understanding and controlling cell metabolism, viability, and productivity, all of which impact key quality attributes such as glycosylation and titer. Relying on delayed nutrient data can hinder timely decision-making and slow process development. High-resolution, real-time analysis better aligns feed strategies with actual cellular demands, reducing variability across scales.

Join Dr. Kenion Blakeman, Principal Scientist, Repligen, as he introduces a new at-line method for amino acid analysis that enables real-time measurements of fresh and spent media directly in the lab. Discover how this approach delivers immediate, high-resolution amino acid profiles, eliminating the need to outsource CE-MS, and supports faster, more informed decision-making. Case studies will demonstrate how immediate access to these profiles is transforming nutrient control, media optimization, and overall process development timelines in both fed-batch and perfusion workflows.

Key learning objectives:

• Learn how real-time amino acid analysis using the REBEL XT has been validated against common cell culture media additives that can interfere with traditional off-line methods.
• Understand how amino acid concentrations vary in response to common cell culture medium modifications and what that means for feed strategy optimization.
• See how the simplicity of the REBEL XT system enables process development scientists, regardless of analytical background, to generate high-quality, at-line data.

Who should attend?

• Upstream process development scientists looking to accelerate media and feed strategy optimization through real-time amino acid data.
• PAT scientists running bioreactors who want to integrate at-line amino acid measurements into process control strategies.
• Analytical scientists and mass spec experts involved in off-line amino acid characterization who are seeking to align on data integrity across lab and process environments.
• Scientists working at the interface of analytical labs and process teams evaluating new technologies to enable faster, more connected decision-making.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.