High parameter spectral flow cytometry: Guiding principles for successful panel design and experiment setup on the ID7000™ Spectral Cell Analyzer

With technology advances in high parameter flow cytometry, researchers are learning how to develop large (20+ parameters) flow cytometry panels for deep immune-profiling studies. The introduction of spectral cytometry analyzers has further accelerated scientists' ability to use complex overlapping fluorochromes, giving them greater flexibility in panel design, and allowing them to consider combinations that were previously challenging to resolve with conventional cytometers.

A good panel design is key to the success of a multicolor flow cytometry experiment, especially as the number of fluorochromes in a high parameter panel increases. Users must take into consideration the spectral properties and brightness of the fluorochromes, the relative expression levels of the antigens, marker co-expression, and commercially available fluorescent antibodies. The attributes of the instrument used for acquiring a high parameter panel also play a key role in the sensitivity and resolution of data.

Join us in this webinar to learn about the key principles of flow cytometry panel design as well as the advantages of utilizing them on the ID7000™ Spectral Cell Analyzer. We will discuss how to apply these principles when developing panels with highly overlapping fluorochromes for spectral flow cytometry. Also, we will review how a high parameter experiment can be optimized for the ID7000 Spectral Cell Analyzer. We will discuss how reference controls can be created, updated, and reused across different experiments, and how the superior optics on the system can be optimized to achieve both high resolution and sensitivity without increasing data spread, to obtain the best quality data.

Key learning objectives

  • Review the principles of panel design and how they can be applied to developing high parameter panels for spectral flow cytometry
  • Understand how spectral flow cytometry panels can be developed to avoid cross-excitation and data spread
  • Understand how key features in the ID7000 Spectral Cell Analyzer such as the reference library, autofluorescence management and instrument standardization can be used to optimize the setup of a high parameter panel to get the highest quality data

Who should attend?

  • This webinar will provide insights to researchers who want to learn how to design immunophenotyping panels for spectral flow cytometry. Attendees will learn about the guiding principles of spectral flow cytometry panel design and how to utilize the tools available in the ID7000 Spectral Cell Analyzer software for spectral reference library setup and instrument optimization for high parameter experiments.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.


Anthony Carcio
Anthony Carcio
Senior Field Application Scientist<p>Sony Biotechnology</p>
Ellen Simms
Ellen Simms
Product and Reviews Editor, SelectScience