Gaining insights into DNA damage response (DDR) in cancer cells with image-based quantification

Maintenance of genome integrity is essential for the prevention of mutations and cellular transformation, which can give rise to cancer. Cancer cells often display repair deficiency which leads to genome instability and can be targeted in cancer therapy. Understanding the detailed molecular mechanism of the DNA damage response (DDR) is therefore essential for the development of much needed new treatment options.

The Spark® Cyto was used to gain insights into DNA repair dynamics in living cells and study the DDR upon treatment with chemotherapeutic agents in fixed cells. Join this webinar to learn how fluorescently tagged proteins can be employed to study their involvement in DNA repair in real-time. Complex cellular responses by cancer cells to genotoxic insults, including post-translational DNA repair specific modifications as well as activation of cell cycle checkpoints will be discussed.

Key learning objectives

  • Learn how to use fluorescently tagged proteins for analysis of repair kinetics
  • Review the workflow used to study the DDR in fixed cells
  • Understand how to generate cell cycle profiles from DNA stains

Who should attend?

This webinar will provide insights to researchers who want to learn about using image-based multiplexing to analyze the phenotypic response to genotoxic insults.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.


Sarah Thomas
Sarah Thomas
Associate Editor, SelectScience
Oliver Mortusewicz
Oliver Mortusewicz
Principal Researcher, Karolinska Institute