Automating large-scale isoform discovery at Seattle Children’s Genomics and Spatial Biology CoLab
Tuesday, September 23, at 17:00 BST | 18:00 CEST | 12:00 EDT | 09:00 PDT
Join Dr. Rebecca Martin, Scientific Lead of the Genomics and Spatial Biology CoLab at Seattle Children’s Research Institute, Dr. Liz Tseng, Product Manager at PacBio, as they discuss how automation is transforming long-read transcriptomics.
Dr. Martin will demonstrate her team’s implementation of PacBio’s Kinnex™ RNA library prep on the SPT Labtech firefly® liquid handler, showcasing how automation enables scalable, reproducible, and high-quality RNA sequencing workflows.
Discover how the Kinnex kit is designed to streamline PacBio’s long-read RNA library prep, preserving full-length transcripts and delivering exceptional isoform resolution. When paired with the firefly® platform, labs can reduce hands-on time and improve consistency across preparations, supporting robust transcriptomic analyses at scale.
PacBio Iso-Seq and Kinnex automation protocols were developed in support of the 1000 Genomes Long Read Project, aiming to produce Iso-Seq data for 1000 diverse samples from the 1000 Genomes cohort. This effort is led by Danny Miller, MD, PhD, Evan Eichler, PhD, at the University of Washington, and Rajiv McCoy, PhD at Johns Hopkins University with support from PacBio and SPT. This open-access dataset will serve as a foundational resource for interpreting gene expression, alternate splicing, and regulatory elements.
Key learning objectives:
- Understand how automation improves long-read RNA sequencing workflows: Learn how integrating PacBio's Kinnex™ RNA library prep kit with the firefly® liquid handler enhances consistency, scalability, and quality in transcriptomic research.
- Explore the benefits of preserving full-length transcripts with long-read sequencing: Gain insight into how Kinnex and PacBio Iso-Seq enable high-resolution isoform detection—crucial for studying gene expression, splicing, and regulatory elements.
- Learn how automated workflows support large-scale transcriptomics projects: See how this automation was applied in the 1000 Genomes Long Read Project to process diverse samples at scale, generating open-access data for the research community.
Who should attend?
- Genomics researchers working on RNA sequencing, transcriptomics, or gene expression studies.
- Core lab managers and automation specialists looking to scale or streamline RNA library prep.
- Molecular biologists focusing on alternative splicing, full-length transcript discovery, or RNA isoform characterization.
- Researchers involved in population-scale genomics or open-data projects.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Speakers
Dr. Rebecca Martin obtained her PhD in Molecular Cell Biology from the University of Washington Department of Genome Sciences. She currently leads the Genomics and Spatial Biology Collaborative Laboratory at Seattle Children's Research Institute. Dr. Martin and the Genomics and Spatial Biology CoLab strive to advance pediatric research by providing access to cutting-edge omics technologies to internal and external researchers.
Liz Tseng is a product manager at PacBio. She obtained her PhD in computer science from the University of Washington and first joined PacBio as a bioinformatics scientist where she developed tools for analyzing long-read RNA Seq data. After transitioning to product marketing, she oversaw the development of the Kinnex kits used for increasing throughput on PacBio sequencers for amplicons.
Melissa Netwal is currently Business Development Scientist at SPT Labtech, where she manages partnerships and collaborations. Prior to joining SPT as a Field Applications Scientist, she was a bench scientist focused on microbial strain and protein engineering.
Moderator
